DNA Genotyping Facility
Description: This is a core facility for allelic discrimination using an ABI 7900HT real time PCR instrument and the TaqMan Assay.
Bill Crider, Ph.D.
Facility Location: McDermott Center for Human Growth & Development
Simmons Biomedical Research Building
6000 Harry Hines Blvd.
Dallas, TX 75390-8591
Pricing: The cost of each SNP assays is $0.30 plus the cost of the ABI probes. The cost of the ABI probes should be around $0.50 per sample. There are several options regarding the ordering of the TaqMan probes from ABI. Probes can be ordered from ABI’s extensive list of pre-designed probes (Assays-By-Design) or ABI can design and test probes (Assays-On-Demand) if your SNP is not included in this list. A third option is to design and order the probes yourself. Each method has associated benefits and costs.
See the ABI home page for more information
Sample Requirements: We need 3 ul of genomic DNA (10 ng/ul) for each SNP you wish to have assayed.