Thermodynamics, Hydrodynamic Properties of Macromolecules
Monday–Friday, May 19–23, 2014
|General Information||Instructional Team|
UT Southwestern Medical Center
T. Boone Pickens Biomedical Building
6001 Forest Park Road
Dallas, TX 75235
See campus map
The workshop will primarily focus on the design, performance, and analysis of solution biophysical experiments. Both hydrodynamic and thermodynamic aspects of macromolecules and their complexes will be covered. The methods overviewed will be sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), dynamic light scattering (DLS), and fluorescence spectroscopy.
The symposium is aimed at researchers with at least limited experience in experimental biophysical methods. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data. Those with some experience with AUC, ITC, DLS, or fluorescence spectroscopy will benefit the most from the workshop.
The format is designed to give the students the maximum exposure to the following methods:
- The first day is devoted to proper experimental practice, including experimental design and hands-on demonstrations of the methods.
- In the subsequent four days, the theoretical aspects of the methods will be reviewed, accompanied by instructor-led practical exercises.
In these exercises, participants will be guided in the used of the software packages SEDFIT, SEDPHAT, NITPIC, and GUSSI to analyze and illustrate the provided data. Users are encouraged to bring their own data, as time will be set aside for one-on-one data-analysis sessions with the instructors. Focus sessions providing a more in-depth treatment of popular topics will also be offered.
This workshop will review strategies for hydrodynamic and thermodynamic analysis of:
- Analytical Ultracentrifugation (AUC)
- Biological macromolecules and their interactions in solution
- Dynamic Light Scattering (DLS)
- Isothermal Titration Calorimetry
- Fluorescence Spectroscopy (anisotropy and quenching isotherms)
- Optical Biosensing (SPR)
Practical data analysis will be demonstrated using the computational tools SEDFIT, SEDPHAT, and EVILFIT.
For more information, please contact Chad Brautigam, Ph.D., Associate Professor of Biophysics, at email@example.com.